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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
24/10/2014 |
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Actualizado : |
15/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
ALEM, D.; DIAZ, P.; LEONI, C.; DE SIMONE, S.G.; CORREA, A.; OPPEZZO, P.; DALLA RIZZA, M. |
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Afiliación : |
DIEGO MARTIN ALEM GLISON, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; PAOLA ALEXANDRA DIAZ DELLAVALLE, Departamento de Ciencia y Tecnología de los Alimentos, Facultad de Ingeniería y Tecnologías, Universidad Católica del Uruguay (UCU), Montevideo, Uruguay; CAROLINA LEONI VELAZCO, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; MARCO DALLA RIZZA VILARO, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
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Título : |
In Search of Topical Agricultural Biofungicides: Properties of the Recombinant Antimicrobial Peptide TrxAq-AMP obtained from Amaranthus quitensis. |
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Fecha de publicación : |
2014 |
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Fuente / Imprenta : |
Journal of Microbial & Biochemical Technology, 2014, v. 6, no.5, p. 268-273. |
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ISSN : |
1948-5948 |
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DOI : |
10.4172/1948-5948.1000155 |
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Idioma : |
Inglés |
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Contenido : |
ABSTRACT.
Synthetic pesticides have a positive impact on food production. However, there are concerns due to the outbreak of resistance along with negative side effects on human health and the environment. New active compounds and control strategies are needed for the management of phytopathogens. Antimicrobial peptides (AMPs) are evolutionarily conserved components of the innate immune response in almost all organisms that constitute an
interesting source of potential molecules for use as pesticides. The naturally derived antimicrobial peptide Aq- AMP (Amaranthus quitensis-Antimicrobial Peptide), obtained from Amaranthus quitensis, is cysteine-rich with activity against several phytopathogens. In the present work, we report on the expression in Escherichia coli of functionally active Aq-AMP fused to thioredoxin (TrxAq-AMP). The in vitro antifungal activity of purified TrxAq-AMP
was confirmed against Alternaria solani, Fusarium oxysporum f. sp. lycopersici, Pencillium digitatum and P. italicum, as well as the in vivo control of P. digitatum in oranges. We demonstrated the stability of TrxAq-AMP in a range of pH (from 3 to 11) and at temperatures from 0°C to 100°C. Furthermore, it maintained activity after digestion with various proteases and it displayed no haemolytic activity suggesting a highly stable and safe molecule. For
topical application we present AMP with no haemolytic/phytotoxic activity that is effective, stable to a wide range of temperature and pH, and resistant to protease activity. Besides, this molecule is naturally stored in the seed, easy to extract and potentially produced through molecular farming. These findings encourage further biotechnological research on topical application of AMPs, especially in relation to molecule bioavailability. MenosABSTRACT.
Synthetic pesticides have a positive impact on food production. However, there are concerns due to the outbreak of resistance along with negative side effects on human health and the environment. New active compounds and control strategies are needed for the management of phytopathogens. Antimicrobial peptides (AMPs) are evolutionarily conserved components of the innate immune response in almost all organisms that constitute an
interesting source of potential molecules for use as pesticides. The naturally derived antimicrobial peptide Aq- AMP (Amaranthus quitensis-Antimicrobial Peptide), obtained from Amaranthus quitensis, is cysteine-rich with activity against several phytopathogens. In the present work, we report on the expression in Escherichia coli of functionally active Aq-AMP fused to thioredoxin (TrxAq-AMP). The in vitro antifungal activity of purified TrxAq-AMP
was confirmed against Alternaria solani, Fusarium oxysporum f. sp. lycopersici, Pencillium digitatum and P. italicum, as well as the in vivo control of P. digitatum in oranges. We demonstrated the stability of TrxAq-AMP in a range of pH (from 3 to 11) and at temperatures from 0°C to 100°C. Furthermore, it maintained activity after digestion with various proteases and it displayed no haemolytic activity suggesting a highly stable and safe molecule. For
topical application we present AMP with no haemolytic/phytotoxic activity that is effective, stable to a wide range of temperature and pH, and resista... Presentar Todo |
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Thesagro : |
ACTIVIDAD ANTIMICROBIANA; ALTERNARIA; BIOTECNOLOGÍA; PÉPTIDOS. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/3627/1/Dalla-Rizza-M.-2014.-Jr.Microb.Biochem.-Technol.-v.65.pdf
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Marc : |
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100 1 $aALEM, D.
245 $aIn Search of Topical Agricultural Biofungicides$bProperties of the Recombinant Antimicrobial Peptide TrxAq-AMP obtained from Amaranthus quitensis.$h[electronic resource]
260 $c2014
520 $aABSTRACT. Synthetic pesticides have a positive impact on food production. However, there are concerns due to the outbreak of resistance along with negative side effects on human health and the environment. New active compounds and control strategies are needed for the management of phytopathogens. Antimicrobial peptides (AMPs) are evolutionarily conserved components of the innate immune response in almost all organisms that constitute an interesting source of potential molecules for use as pesticides. The naturally derived antimicrobial peptide Aq- AMP (Amaranthus quitensis-Antimicrobial Peptide), obtained from Amaranthus quitensis, is cysteine-rich with activity against several phytopathogens. In the present work, we report on the expression in Escherichia coli of functionally active Aq-AMP fused to thioredoxin (TrxAq-AMP). The in vitro antifungal activity of purified TrxAq-AMP was confirmed against Alternaria solani, Fusarium oxysporum f. sp. lycopersici, Pencillium digitatum and P. italicum, as well as the in vivo control of P. digitatum in oranges. We demonstrated the stability of TrxAq-AMP in a range of pH (from 3 to 11) and at temperatures from 0°C to 100°C. Furthermore, it maintained activity after digestion with various proteases and it displayed no haemolytic activity suggesting a highly stable and safe molecule. For topical application we present AMP with no haemolytic/phytotoxic activity that is effective, stable to a wide range of temperature and pH, and resistant to protease activity. Besides, this molecule is naturally stored in the seed, easy to extract and potentially produced through molecular farming. These findings encourage further biotechnological research on topical application of AMPs, especially in relation to molecule bioavailability.
650 $aACTIVIDAD ANTIMICROBIANA
650 $aALTERNARIA
650 $aBIOTECNOLOGÍA
650 $aPÉPTIDOS
700 1 $aDIAZ, P.
700 1 $aLEONI, C.
700 1 $aDE SIMONE, S.G.
700 1 $aCORREA, A.
700 1 $aOPPEZZO, P.
700 1 $aDALLA RIZZA, M.
773 $tJournal of Microbial & Biochemical Technology, 2014$gv. 6, no.5, p. 268-273.
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Registro original : |
INIA Las Brujas (LB) |
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| Registros recuperados : 102 | |
| 81. |  | BANCHERO, G.; LA MANNA, A.; FERNANDEZ, E.; TIERI, M.; MIERES, J.; PÉREZ, J.; UZUCA, J.; PÉREZ, E. Pastoreo de verdeos de invierno y necesidades de consumo de agua, en terneros post destete ln: INIA LA ESTANZUELA. Jornada de Invernada Intensiva, julio, La Estanzuela, Colonia, Uruguay, 2010. Producción de carne desde una invernada de precisión. La Estanzuela, Colonia (Uruguay): INIA, 2010. p. 35-37 (INIA Serie Actividades de Difusión ; 609)| Biblioteca(s): INIA Tacuarembó. |
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| 82. | | LEMA,O.M.; BRITO, G.; CLARIGET, J.; PEREZ, E.; LA MANNA, A.; BANCHERO, G.; RAVAGNOLO, O.; MONTOSSI, F. Performance and carcass quality of steers with different nutritional levels and parental EPD for rib eye area. In: INTERNATIONAL CONGRESS OF MEAT SCIENCE AND TECHNOLOGY, 60. , 2014, Punta del Este, Uruguay: ICOMST. 2014. Archivos Latinoamericanos de Producción Animal, 2014, v.2 ,no.5.p. 652-655.| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA La Estanzuela. |
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| 83. | | FERNÁNDEZ, G.; SBRES, M.; LADO, J.; PEREZ, E. Postharvest sour rot control in lemon fruit by natamycin and an Allium extract. International Journal of Food Microbiology, 2022, Volume 368, Article number 109605. doi: https://doi.org/10.1016/j.ijfoodmicro.2022.109605 Article history: Received 9 November 2021; Received in revised form 15 February 2022; Accepted 25 February 2022; Available online 1 March 2022.
Corresponding author: Pérez-Faggiani, E.; Programa Nacional de Investigación en Producción...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| 85. | | FERNANDEZ, E.; LANFRANCO, B.; BANCHERO, G.; PEREZ, E.; LA MANNA, A.; CANOZZI, M.E.A.; CLARIGET, J.M. Producción y eficiencia en sistemas ganaderos intensivos. Socio-Economía. Revista INIA Uruguay, Marzo 2022, no.68, p.31-35. (Revista INIA; 68).| Tipo: Artículos en Revistas Agropecuarias |
| Biblioteca(s): INIA Las Brujas. |
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| 86. | | SILVERA-PÉREZ, E.; MAESO, D.; CATARA, V.; RUBIO, L.; LEONI, C.; AMARAL, J.; ESTELDA, C.; HERNÁNDEZ, M.; BÓFFANO, L.; GONZÁLEZ, P. Pseudomonas spp. associated with tomato pith necrosis in the Salto area, Northwest Uruguay. European Journal of Plant Pathology, 2023, volume 165, issue 4, pp.715-724. doi: https://doi.org/10.1007/s10658-023-02639-6 Article history: Accepted 10 January 2023; Published 19 January 2023. -- Corresponde author: Silvera-Pérez, E.; Plant Protection Department, Facultad de Agronomía, Universidad de la República, Route 31 km 21.5, Salto, Uruguay;...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| 94. | | CANOZZI, M.E.A.; CLARIGET, J.M.; ROIG, G.; PEREZ, E.; AZNÁREZ, V.; BANCHERO, G.; LA MANNA, A. Shade effect on behaviour, physiology, performance, and carcass weight of heat-stressed feedlot steers in humid subtropical area. Animal Production Science, 2022, Volume 62, Issue 17, p.1692-1705. Doi: https://doi.org/10.1071/AN22128 Article history: Submitted: 13 October 2021; Accepted: 3 June 2022; Published online: 12 July 2022. -- Correspondence author: La Manna, A.; Instituto Nacional de Investigacíon Agropecuaria, INIA La Estanzuela, Ruta 50 km 11, Colonia,...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA La Estanzuela. |
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| 96. | | CANOZZI, M.E.A.; ZARZA, R.; BANCHERO, G.; LA MANNA, A.; FERNANDEZ, E.; PEREZ, E.; CLARIGET, J.M. Suplementación estival de novillos sobre año en pastoreo. Revista INIA Uruguay, Diciembre 2022, no.71, p.25-28. (Revista INIA; 71)| Tipo: Artículos en Revistas Agropecuarias |
| Biblioteca(s): INIA Treinta y Tres. |
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| 97. | | PEYROU, M.; RIGAMONTI, N.; DEL CAMPO, R.; RUSSI, P.; LARRECHART, L.; PEREZ, E.; MARA, H. Técnicas biológicas, serológicas y moleculares para la detección asintomática de Xanthomonas axonopodis pv. citri Agrociencia Uruguay, 2012, v.16, no.1, p.124-133| Tipo: Artículos en Revistas Indexadas Nacionales | Circulación / Nivel : B - 2 |
| Biblioteca(s): INIA La Estanzuela; INIA Las Brujas. |
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| 98. | | CLARIGET, J.M.; PEREZ, E.; UZUCA, J.; BATISTA, E.; GONNET, R.; CANOZZI, M.E.A. TPP 28. Efecto de la caminata pre-faena sobre el peso de la canal caliente en novillos de razas británicas. [resumen]. [Effect of pre-slaughter walking on hot carcass weight in British steers]. [abstract] Tecnología de Productos Pecuarios. En: Congreso Argentino de Producción Animal Virtual, 45, 16 al 18 de noviembre 2022. p.249. (Revista Argentina de Producción Animal ; 42 Suplemento 1). Corresponding author: Clariget, J.M. Instituto Nacional de Investigación Agropecuaria (INIA, Uruguay). E-mail: jclariget@inia.org.uy| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA Las Brujas. |
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| 99. | | AVANCE ANUAL DE LA INVESTIGACIÓN EN POSCOSECHA DE CÍTRICOS. JORNADA DE DIVULGACIÓN, DICIEMBRE 2011, SALTO GRANDE (UY) - VARELA, P.; LADO, J.; PÉREZ, E.; BESIL, N.; PÉREZ, A.; CESIO, V.; HEINZEN, H. Trabajos presentados Salto (Uruguay): INIA Salto Grande, 2011. 22 p. (INIA Serie Actividades de Difusión; 667) INIA, Programa Nacional de Investigación en Producción Citrícola| Tipo: Actividades de Difusión |
| Biblioteca(s): INIA Las Brujas; INIA Tacuarembó. |
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| Registros recuperados : 102 | |
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